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. 2006 Oct 3;103(41):15136–15141. doi: 10.1073/pnas.0607181103

Fig. 3.

Fig. 3.

IRF1-dependent IFN-β induction in GM-DCs. (a) GM-DCs derived from wild-type or mutant mice were stimulated with 0.3 μM CpG-B, 100 ng/ml LPS, or 100 μg/ml poly(I:C) for the indicated periods. Total RNA was prepared and subjected to real-time RT-PCR analysis. (b) Wild-type or mutant GM-DCs were unstimulated (gray) or stimulated with 100 ng/ml LPS (blue) or 0.3 μM CpG-B (red) for 18 h and analyzed for CD40 expression by flow cytometry. (c) Splenic pDCs from wild-type or mutant mice were stimulated with CpG-A (3 μM) and subjected to real-time RT-PCR analysis using IFN-α1 or IFN-β primers.