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IRF1 involved in IFN-γ and TLR synergy. GM-DCs from wild-type or mutant mice were pretreated with or without 200 units/ml IFN-γ for 4 h and then stimulated with 0.3 μM CpG-B (a) or MALP2 (b) for the indicated periods. Total RNA was prepared and subjected to real-time RT-PCR analysis of the indicated genes.
