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Effect of synergism between IFN-γ and TNF-α. GM-DCs from wild-type mice were pretreated with or without 200 units/ml IFN-γ for 4 h and then stimulated with 0.3 μM CpG-B or 10 ng/ml TNF-α for the indicated periods. Total RNA was prepared and subjected to real-time RT-PCR analysis of the indicated genes.
