Figure 3.

Expression of Apaf-1⁵³⁰ induces caspase 9-dependent reporter activation. W303α containing pSH18–34 and pGALL-CLBDG6 were transformed with pGALL plasmids to carry either two empty pGALL vectors, Apaf-1⁵³⁰ and an empty pGALL vector, caspase 9 and an empty pGALL vector, or Apaf-1⁵³⁰ and caspase 9. Two colonies from each transformation were streaked onto selective glucose medium plates, were grown for several days, and then were replica plated onto complete gal/raf medium. After 16 hr, an X-gal assay was performed. Only cells expressing both Apaf-1⁵³⁰ and caspase 9 show significant β-gal activity.