Methylation status of CpG islands corresponding to clones pSPM210 and pSPM814. Genomic DNA derived from a cancerous and noncancerous portion of the same patients was digested, fractionated on an MBD column, amplified by PCR, and applied to agarose gel. Numbers indicate a specific patient. C, fractionated template DNA from cancerous portion; N, from apparent noncancerous portion of the lung; M, HaeIII digest of pUC19; 1, flow-through fraction; 2, lower salt fractions (0.40–0.56 M NaCl); 3, higher salt fractions (0.60–1.0 M NaCl).