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Methylation status of CpG islands corresponding to fragments pSPM210 and pSPM814 in cultured cancer cells. (A) M, HaeIII digest of pUC19; 1, flow-through fraction; 2, lower salt fractions (0.40–0.48 M NaCl); 3, higher salt fractions (0.52–1.0 M NaCl) (B) Southern hybridization experiment using the 0.4-kb ApaI/BstXI insert of clone pSPM210 as a probe. 1, Tsp509I digest; 2, Tsp509I/MspI digest; 3, Tsp509I/HpaII digest.
