Table 3.

Rescue of daf-18(e1375) by an 8-kb subclone of cosmid T07A9

Genotype	Rol animals, no.*		Non-rol, no.†
	Dauers	Adults/L4	Dauers	Adult/L4
daf-2	—	—	134	0
daf-2; daf-18	—	—	3‡	392
daf-2; daf-18; array 1	21	0	0	16
daf-2; daf-18; array 2	37	0	5	21
daf-2; daf-18; array 3	33	0	1§	9
daf-2; daf-18; array 4	7	0	3	15

Adults hermphrodites (n = 4–6) were allowed to lay eggs for 5–6 h. Progeny were placed at 25°C and scored visually 78 h after eggs were laid. These data are from one representative experiment. Experiments were performed at least twice. 

^*Transgenic animals were marked with the rol-6 transgene. 

^†Non-roll animals are largely not transgenic, although a small proportion may be mosaic for the extrachromosomal array. 

^‡These were “partial dauers.” They were lighter and moved more than true dauers. 

^§This animal looked like an L2d larva, because it was darker than a normal L2 larva.