Table 1.
DNA | Transformants-PCR products
|
Cut patterns of the long bands
|
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Distal cuts
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Proximal cuts
|
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Total | Short | Long | Δ TE* | AflII | NdeI | 5′, % | HindIII | ClaI | 5′, % | |
HetI | 231 | 73 | 158 | 0.32 | 70 (5′) | 21 (3′) | 77 | 58 (5′) | 9 (3′) | 87 |
HetII | 217 | 74 | 143 | 0.34 | 20 (3′) | 63 (5′) | 76 | 11 (3′) | 49 (5′) | 82 |
Transformation efficiency of the deletion—i.e., the ratio short bands/total.