Table 4.
Analysis of the transformants obtained with circular heteroduplexes in R317: recA+(Ind−), hex−
| DNA | Transformants-PCR products
|
Cut patterns of the long bands
|
|||||||
|---|---|---|---|---|---|---|---|---|---|
| Total | Short | Long | Δ TE | AflII | NdeI | HindII | ClaI | 5′, % | |
| HetI | 88 | 11 | 77 | 0.12 | 36 (5′) | 5 (3′) | 28 (5′) | 8 (3′) | 83 |
| HetII | 30 | 4 | 26 | 0.13 | 1 (3′) | 13 (5′) | 2 (3′) | 10 (5′) | 88 |