Figure S3. ADP Product Inhibition (Vary Tide).

Individual direct plots of k versus [Tide]. The concentration of Tide was varied at six different concentrations of ADP. ADP inhibition studies were carried out at seven different concentrations of ATP [0.3 μM (•), 1 μM (○), 3 μM (▪), 10 μM (□), 30 μM (▴), 100 μM (▵), and 300 μM (▾)]. Dashed lines indicate direct fits of each row of individual data sets to the general velocity equation for noncompetitive inhibition, which yielded values of ^appk_cat, ^appK_m^ATP, and ^appK_i^ADP. Solid lines indicate the global fit of these data to Equation 6, which describes a Rapid Equilibrium Random Bi Bi system with a E-ADP-Tide dead-end ternary complex (Fig. 4G).