Table 1.
STS sequences and PCR conditions for MyHC contigs
| STS/Gene name | Primer Sequence 5′ → 3′ | Anneal temp.,°C | Product size, bp |
|---|---|---|---|
| A. Human | |||
| MyHC-emb5′ | F-GATGTGGTTCCAGCACTGG | ‡58 | 174 |
| R-CTGGCCTACAGTGAACGTG | |||
| MyHC-emb3′ | F-GCCAGCCCTTCTGGAGCA | 55 | 93 |
| R-GAGTCACATGGACATTAAGT | |||
| †MyHC-IIa5′ | F-TGTCGGGACTCTCAGGTAG | 58 | 200 |
| R-CAGCATCTATTAGCGTGTCC | |||
| MyHC-IIa3′ | F-TCATGTCCTGATGCCATGGAATG | 63 | 119 |
| R-CTACCCTATGCTTTATTTCCTTTG | |||
| *A288E1L | F-TAGCCATTGCCTATATCAAG | 55 | 108 |
| R-GCATCGAGCTCATCGAGAAG | |||
| MyHC-IIx/d3′ | F-ATCTAACTGCTGAAAGGTGACC | 56 | 71 |
| R-TAAGTACAAAATGGAGTGACAAAG | |||
| MyHC-IIb5′ | F-CCTAAGTACTGTGTTAGTTCTCAT | 65 | 1,000 |
| R-TCTGAACATGCTTGGAGCATCA | |||
| MyHC-IIb3′ | F-GTTATCTCAAATGCATCTCTGTTC | 66 | 150 |
| R-GATATACAGGACAGTGACAAAGAACT | |||
| MyHC-pn5′ | F-CAGGGAGAAAATACTCCC | 58 | 171 |
| R-GTTGGGAAGGGAAGACATG | |||
| MyHC-pn3′ | F-TATCAAGAGGCTGAAGAAAG | 47 | 112 |
| R-AATGTATACATTTACTGTAG | |||
| MyHC-eo5′ | F-GAGCATGCCCATATTATCAAAGAC | 67 | 300 |
| R-TGCAGTCATGAGCTCTGAGCGA | |||
| †401014T7 | F-CCCTTCTGGTTCTGCAGACT | 57 | 73 |
| R-CGGTCATAGAGAAGACCAGAGA | |||
| MyHC-eo3′ | F-CATGAGCTAGAGGAGGCCGC | 63 | 885 |
| R-GTATTTAGGAGAATTTATTTCTCA | |||
| B. Mouse | |||
| †475-D-1T7 | F-ATGAACTGGAAACCCTCCTC | 56 | 158 |
| R-CTTGAGAAGCTGAAGTAGTAG | |||
| †538-C-3T7 | F-GTATCTCCAGCACCCTAC | 50 | 276 |
| R-TGATGGAGTCACAAAGATCAC | |||
| MyHC-emb3′ | F-TGAGCAAGACCTCCTGGTG | 56 | ∼100 |
| R-TGCATGTGGAAAAGTGATACG | |||
| MyHC-IIa5′ | F-CTGTACAAAGATCTGCTGT | 50 | ∼300 |
| R-CAAGTCAGCGAGTGACCA | |||
| MyHC-IIa3′ | F-GGCGAGGCACAAAATGTGAA | 56 | ∼100 |
| R-GCAAAGGAACTTGGGCTCTT | |||
| MyHC-IIx/d5′ | F-ATTTCTCTCTCTGCCAGGCA | 56 | ∼200 |
| R-TCAAACTAGCCCCGCAGTG | |||
| †380-N-16T7 | F-TGGACAAGGGTATGAACTTCG | 56 | 120 |
| R-GAGACATTGATGGGTCCTTG | |||
| MyHC-IIx/d3′ | F-ATTGATCCAAGTGCAGGAAAG | 56 | ∼200 |
| R-TCCTGATCTACAAATGATCGG | |||
| †630-D-9T7 | F-CTTAGCAGGTCTCCACGTC | 56 | 328 |
| R-GTGATGCTTGCAAAGGAGTC | |||
| MyHC-IIb5′ | F-TCTGTCACTCGGTGCTTCC | 56 | ∼200 |
| R-AGGGTTTTTGGAGGCTGTTT | |||
| †433-B-7T7 | F-GTCGTGCCTAAGTCCTAGC | 56 | 109 |
| R-AGGCCCAAGAGAGAATTTGC | |||
| †561-O-1T7 | F-CTGTCCACCTACCTTGAGG | 56 | 366 |
| R-GAGTTCTCTTCTGCTCAGTG | |||
| MyHC-pn3′ | F-ACACATCTTGCAGAGGAAGG | 56 | ∼100 |
| R-TAAACCCAGAGAGGCAAGTG | |||
| †386-N-23T7 | F-GCACTGTTAACCGTGAGCC | 56 | 185 |
| R-GGTAAATCAAACCAGGCTCC | |||
| †645-M-6T7 | F-AGAACGAGGAAAAGCAAGGC | 56 | 218 |
| R-CAGATATTCCTTCTGGGCAC | |||
| †410-O-9T7 | F-AGTTCACTGCCCTAAGCATG | 56 | 177 |
| R-AAGGCAGCTGTGGATATGAC | |||
| MyHC-eo3′ | F-CAGGTAAAAGCAGACACCTG | 56 | ∼300 |
| R-AGCTTTAAGGAATAGGCTTCG | |||
| †524-K-7T7 | F-AACATTGATGACTGCCCTGC | 56 | 299 |
| R-TTGGAGGTAGAGTTGTCAGG |
*, STS used in YAC contig (19), (†) STS from PAC End Rescue. All PCR used 1.5mM MgCl2 (detailed in Materials and Methods) except (‡) 2.0mM MgCl2.