Table 1.

Complementation of msh2 mutator phenotype by the wild-type MSH2 gene on a plasmid

Strain	Gene in the vector	Promotor	Vector	Mutation rate × 106	Confidence limits	Relative rate
MSH2*	None	—	None	0.16	0.12–0.24	1
msh2*	None	—	None	1600	1,400–2,100	10,000
msh2	None	GAL10	2μ	620	530–1,900	8,800
	MSH2	GAL10	2μ	0.07	0.03–0.2	1
	MSH2	MSH2	ARS-CEN	0.64	0.4–1.20	9
MSH2	None	GAL10	2μ	0.14	0.06–0.4	1
	MSH2	GAL10	2μ	0.15	0.12–0.25	1
	MSH2	MSH2	ARS-CEN	0.15	0.06–0.2	1

Mutation rates were obtained for (typically) 12 independent colonies. The respective vector construct without the MSH2 gene was not available for the ARS-CEN plasmid. 

^*The mutation rates for these strains have already been described (32).