Table 3.
Dominant negative mutator effects of msh2 P-loop mutants in an MSH2 strain
| MSH2 allele in the vector |
GAL10 inducible promotor 2μ plasmid
|
MSH2 promotor ARS-CEN plasmid
|
||||
|---|---|---|---|---|---|---|
| MR × 106 | CL × 106 | RR | MR × 106 | CL × 106 | RR | |
| None | 0.14 | 0.1–0.4 | 0.9 | ND | ||
| MSH2 | 0.15 | 0.12–0.25 | 1 | 0.15 | 0.06–0.2 | 1 |
| G693S | 1,400 | 1,200–2,000 | 9,300 | 1.1 | 0.8–1.4 | 7 |
| G688A | 3 | 0.2–11 | 20 | 1.2 | 0.6–4.7 | 8 |
| G693A | 1,050 | 900–2,600 | 7,000 | 5.1 | 1.5–6.7 | 34 |
| K694A | 920 | 600–2,600 | 6,100 | 0.6 | 0.3–1.8 | 4 |
| S695A | 4,100 | 1,800–7,400 | 27,000 | 0.6 | 0.2–1.1 | 4 |
MR, median Lys+ reversion rates for (typically) 12 individual colonies; CL, 95% confidence limits; RR, relative rate, in reference to wild type; ND, not determined.