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Lentiviral vector expression in CD34+CD38− cells defined according to cell cycle status. Cell cycle analysis of cells derived from the CD34+CD38− gate (not shown) and simultaneous GFP expression of G0, G1, and S/G2/M populations are shown. Black histogram = transduced cells, gray histogram = nontransduced cells (negative control). Percent GFP+ was calculated by subtracting the negative control cells falling within the marker region. A second experiment yielded similar results.
