Vector expression in transduced CD34+ and CD34+CD38− cells. FACS analysis was performed on LTC of transduced cells at the time points indicated. Mean ± SEM for all experiments is shown. For all panels, the legend of vectors is as follows: lenti/VSV = solid line with ○; MLV/GALV = dashed line with □, and MLV/VSV = broken-dashed line with ▵. (A) CD34+ cells transduced with growth factors by lenti/VSV (n = 11), MLV/GALV (n = 6), and MLV/VSV (n = 5). (B) CD34+ cells transduced in the absence of growth factors by lenti/VSV (n = 11), MLV/GALV (n = 8), and MLV/VSV (n = 7). (C) CD34+CD38− cells transduced with growth factors by lenti/VSV (n = 7), MLV/GALV (n = 4), and MLV/VSV (n = 4). (D) CD34+CD38− cells transduced in the absence of growth factors by lenti/VSV (n = 4), MLV/GALV (n = 4), and MLV/VSV (n = 2).