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. 1999 Mar 16;96(6):3000–3005. doi: 10.1073/pnas.96.6.3000

Figure 2.

Figure 2

Class-switching defects in Iγ2b N/N and IgE N/N mice. (Upper) Concentrations of specific Ig isotypes in sera from Iγ2b−/− (□), Iγ2bN/N (⋄), Iγ2b +/N (○), wt (TC1/WT) (▵), and IgE N/N (♦) mice as measured by ELISA. Serum from some neor-inserted mice contained undetectable levels of IgG3 (symbols on axis). (Lower) ELISA analyses of culture supernatants from splenic B cells from Iγ2b−/− (squares), Iγ2bN/N (diamonds), Iγ2b +/N (circles), wt (TC1/WT) (triangles), or IgE N/N (◃) mice stimulated in vitro for 4.5 days in the presence of LPS (open symbols) (IgM, IgG3, IgG2b), LPS plus interferon γ (closed symbols for IgG2a), LPS plus IL-4 (closed symbols for IgG1 and IgE), or LPS plus transforming growth factor β (closed symbols for IgA). For Iγ2bN/N cultures, IgG3 was reduced 40- to 200-fold and IgG1 was partially inhibited (reduced 10-fold) compared with controls. For IgEN/N cultures, IgG3 was down 40- to 200-fold, IgG2b was down 20- to 50-fold, IgG2a was down 5- to 20-fold, IgG1 was reduced slightly (down 10-fold), and IgE was reduced severely.