Figure 3.

Generation of CyCAP −/− mice. (A) The CyCAP genomic organization (Top), targeting vector (Middle), and targeted locus (Bottom). Exons are numbered 1–6, and restriction-enzyme sites are indicated. B, BamHI; H, HpaI. The expected size fragments for BamHI-digested DNA hybridized with the indicated 5′ probe for wild-type and targeted alleles are shown. (B) Southern blot analysis of representative tail DNA. Genomic tail DNA was digested with BamHI and hybridized with the 5′ probe indicated in A.