Figure 4.

CyCAP −/− mice do not express CyCAP protein or RNA. (A) CyCAP is secreted from macrophages from wild-type (+/+) but not CyCAP-deficient (−/−) mice. Medium from metabolically labeled bone-marrow-derived macrophages was incubated with rat polyclonal serum to CyCAP (α-CyCAP) or normal rat serum (NRS). (B) CyCAP RNA is expressed in CyCAP +/+ but not CyCAP −/− mice. Northern blot analysis of total RNA from kidney and liver of wild-type and CyCAP-deficient mice probed sequentially with full-length ³²P-labeled CyCAP and cypA cDNA probes.