Figure 7.

TNF-α release from PECs stimulated with LPS in the presence of serum from wild-type or CyCAP-deficient mice. (A) PECs from CyCAP-deficient mice were pooled and stimulated in vitro for 6 h with indicated concentrations of LPS in the presence of serum from CyCAP −/− (diamonds) or wild-type (squares) mice. TNF-α values represent means of duplicate samples ± SD. (B) PECs from eight CyCAP-deficient mice (cell numbers from 1 × 10⁶ to 5 × 10⁶ cells per ml) were stimulated with LPS (0.1 μg/ml) in serum from CyCAP +/+ or −/− mice, and TNF-α levels were measured. Data were analyzed by using a paired t test; P = 0.002.