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. 1999 Mar 16;96(6):3017–3022. doi: 10.1073/pnas.96.6.3017

Figure 1.

Figure 1

Binding of COS cells expressing ICAM-2 to immobilized LFA-1 and effect of mAbs. (A) COS cells were transfected with 0, 0.1, 0.375, 0.75, 1.5, 3, 6, or 8 μg of ICAM-2 cDNA. The percentage of cells binding to purified LFA-1 adsorbed to plastic (y-axis) and expressing ICAM-2 by using the anti-ICAM-2 antibody CBR-IC2/1 and immunofluorescence flow cytometry (x-axis) was determined. Data are average and standard deviation for 10 different experiments. (B) Binding to LFA-1 of COS cells transfected with ICAM-2 or mock transfected with AprM9 was determined in the presence of 10 μg/ml of anti-ICAM-2 antibodies CBR-IC2/1, CBR-IC2/2, BT-1, and 6D5, anti-ICAM-1 mAb RR1/1, and LFA-1 β-subunit mAb TS1/18, respectively. The amount of binding to LFA-1 is expressed as a percentage of the binding obtained in the absence of mAb. Data are for an average of two to five experiments.