Figure 5.

Reduced production of IL-4 after stimulation with ICAM-1-expressing Drosophila APC is apparent at the level of mRNA. D011 cells were cultured with 10 μM Ova peptide presented by either T-S or Drosophila APC expressing the indicated accessory molecules. RNA purified after 24, 48, and 72 hr of culture was assessed for cytokine gene expression by using a multiprobe RNase protection assay (as described). (A) A representative autoradiograph for IL-4, IL-2, and the housekeeping gene L32, is shown. (B) The radioactivity in the bands was quantitated by using a PhosphorImager and the results plotted as percentage of L32.