Figure 2.

Processing of the mutant A4CT proteins to Aβ. COS7 cells were transfected stably with the indicated SPA4CT constructs (the numbers indicate the residue in the A4CT-sequence that was replaced by phenylalanine) and metabolically labeled with [³⁵S]methionine. Aβ was immunoprecipitated from the conditioned medium by using the antibodies shown on the left side of the gels. W02, an antibody that binds all Aβ species; 40, antibody G2–10 that binds Aβ₄₀ and p3₄₀; 42, antibody G2–11 that binds Aβ₄₂ and p3₄₂; C, vector control. Vertical black bars distinguish the two separate electrophoresis gels used.