Figure 5.

Autophosphorylation of VEGFRs after stimulation by recombinant ORFV 2-VEGF. PAE cells expressing VEGFR-2 or NIH 3T3 cells expressing VEGFR-3 were made quiescent by starvation overnight in growth medium containing 0.2% BSA. The cells were stimulated with either ORFV 2-VEGF (100 ng/ml), VEGF₁₆₅ (50 ng/ml), VEGF-CΔNΔC (100 ng/ml), or mock medium, lysed, and immunoprecipitated by using receptor-specific antibodies. The immunoprecipitates were analyzed by using phosphotyrosine immunoblotting. The apparent molecular weights of the tyrosyl phosphorylated VEGFR-2 and VEGFR-3 polypeptides are shown.