Figure 7.

Vascular permeability activity of ORFV2-VEGF in the Miles assay. Anesthetized guinea pigs were given intracardiac injections of Evan’s Blue dye. Purified ORFV2-VEGF, mouse VEGF₁₆₄, and appropriate controls were diluted in medium and applied (150 μl) intradermally to shaved areas on the back of the animal. After 30 min, the animals were sacrificed, and the skin was excised (A) and eluted in formamide, and the absorbance reading at 620 nm (B) was recorded. Values are expressed as mean ± SD and are representative of three experiments.