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Cellular localization of Hdm2 mutants. 2KO and H1299 cells were (co)transfected with the expression plasmid(s) encoding Hdm2 mutants as indicated above the pictures. Cellular localization of Hdm2 mutants in 2KO (a–c) or H1299 (d–f) cells was determined by using immunofluorescence 24 hours after transfection as described in Materials and Methods.
