Figure 4.

Coexpression of either Hdm2NLS or Hdm2NES with wild-type Hdm2 has no effect on Hdm2’s ability to promote p53 degradation. 2KO cells were cotransfected with p53 expression plasmid and the Hdm2 plasmid alone or with the plasmid encoding its mutant (as indicated). At 24 hours after transfection, steady-state levels of p53 was determined by using immunoblotting as described in Materials and Methods.