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. 2006 Sep 14;25(19):4628–4637. doi: 10.1038/sj.emboj.7601327

Figure 4.

Figure 4

ScpC is responsible for degradation of KC and MIP-2 in infected skin. (A) KC levels (ng/mg protein) extracted from skin lesions of mice inoculated with WT (Inline graphic), ΔscpA (▵), and ΔscpA/ΔscpC (▴). Each point represents mean of the determinations performed on three mice killed at the indicated time points after infection. Assays by ELISA were conducted in duplicate. Error bars represent s.d. P<0.05 (Student's test) of ΔscpA/ΔscpC versus WT or versus ΔscpA for each time point. P>0.05 (Student's test) for WT versus ΔscpA. (B) MIP-2 levels (ng/mg protein) extracted from skin lesions of mice inoculated with WT (Inline graphic), ΔscpA (□), and ΔscpA/ΔscpC (▪). Each point represents mean of the determinations performed on three mice killed at the indicated time points after infection. Assays by ELISA were conducted in duplicate. Error bars represent s.d. P<0.01 (Student's test) of ΔscpA/ΔscpC versus WT or versus ΔscpA. P>0.05 (Student's test) for WT versus ΔscpA for each time point. (C) LIX levels (ng/mg protein) extracted from skin lesions of mice inoculated with WT (Inline graphic), ΔscpA (○), and ΔscpA/ΔscpC (•). Each point represents mean of the determinations performed on three mice killed at the indicated time points after infection. Assays by ELISA were conducted in duplicate. Error bars represent s.d. P>0.05 (Student's test) of ΔscpA/ΔscpC versus WT or versus ΔscpA. P>0.05 (Student's test) for WT versus ΔscpA for each time point. (D) KC levels (ng/spleen) in spleens of three mice from the same groups of mice described in panel A. Assays by ELISA were conducted in duplicate. Error bars represent s.d. P>0.05 (Student's test) of ΔscpA/ΔscpC versus WT or versus ΔscpA for each time point. (E) MIP-2 levels (ng/spleen) in spleens of three mice from the same groups of mice described in panel B. Assays by ELISA were conducted in duplicate. Error bars represent s.d. P>0.05 (Student's test) of ΔscpA/ΔscpC versus WT or versus ΔscpA for each time point. (F) KC levels (ng/ml) in sera of three mice from the same groups of mice described in panel A. Assays by ELISA were conducted in duplicate. Error bars represent s.d. P>0.05 (Student's test) of ΔscpA/ΔscpC versus WT or versus ΔscpA for each time point. (G) MIP-2 levels (ng/ml) in sera of three mice from the same groups of mice described in panel B. Assays by ELISA were conducted in duplicate. Error bars represent s.d. P>0.05 (Student's test) of ΔscpA/ΔscpC versus WT or versus ΔscpA for each time point.