Figure 2.

ER tubules are transported into newly developed ptc1Δ buds, but display a dramatic delay in their delivery to the bud cortex. Time-lapse images of wild-type (SFNY1625) and ptc1Δ (SFNY1624) diploid cells grown in SC medium at 25°C were acquired at 5 min intervals during 120-min time period as described in the Materials and methods. Ten cells were analyzed for each strain and the dynamics of the ER in representative cells are shown. These selected frames from the time series demonstrate that the release of ER tubules from the bud tip is severely delayed in ptc1Δ buds. Bar, 5 μm.