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. 2006 Sep 14;25(19):4557–4566. doi: 10.1038/sj.emboj.7601307

Figure 7.

Figure 7

p97 facilitates membrane extraction independently of cleavage. TMD1 degradation reactions were carried out in (A) mock- or (B) p97-depleted RRL, and cytosolic supernatants were analyzed for CFTR fragments before (total released) or after (TCA sol) TCA precipitation. Vertical arrow indicates TCA-insoluble peptide fragments that accumulate in the supernatant. (C) Autoradiograms showing dislocated full-length TMD1 degradation products generated in the presence of MG132 (lanes 1–10), in mock-depleted (lanes 1–5) or p97-depleted (–p97, lanes 6–10) RRL. No fragments were visualized by SDS–PAGE in the absence of either ATP (lanes 11–16) or MG132 (lanes 16–20). The extent (D) and initial rate (E) of total TMD1 released in the presence of MG132 in mock-depleted (Ni), p97-depleted (depl.) or depleted RRL supplemented with recombinant p97 (depl.+p97).