Figure 1.

Genotyping strategy of mice harboring germ-line mutations in p53 and/or decorin. (Upper) Schematic representations of the wild-type and targeted alleles and the primer position for the p53 and decorin genes, respectively. (Lower) Representative PCR products used to genotype animals derived from the mating of p53−/− and Dcn−/− mutant mice. The germ-line mutation of p53 and Dcn consists of an insertion of PolII-neo or PGK-neo in the fifth and second exon of their respective genes. Three separate sets of PCR amplifications were performed for each individual offspring and confirmed by DNA sequencing. The genotype is indicated at the top of the ethidium bromide-stained agarose gels, and the molecular weight in bp is indicated in the right margins.