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. 1999 Mar 16;96(6):3092–3097. doi: 10.1073/pnas.96.6.3092

Figure 4.

Figure 4

Decorin inhibits the growth of PD100 thymic lymphoma cells derived from p53−/− Dcn−/− animals. (A) Western immunoblotting of serum-free medium conditioned by embryonic fibroblasts derived from Dcn+/+ and Dcn−/− mouse embryos (MEF) using LF-113 polyclonal antibody directed against a synthetic peptide in the N terminus of decorin spanning residues 36–49 of the mouse protein core (24). The migration of the molecular mass markers is indicated in the left margin. Under such conditions, decorin migrates as a smear centering around 100 kDa (10). (B) Growth of PD100 thymic lymphoma cells in the presence or absence of a feeder layer composed by mitomycin-arrested MEFs. The genotype is indicated in the top margin. (C and D) Growth of PD100 thymic lymphoma cells cultured in the absence or presence of recombinant human decorin or its protein core (1 μM each). Cells were counted after 60 hr of culture in 5% FBS. The values represent the mean of quadruplicate determinations ±SEM.