Figure 5.

Inhibition of PD100 colony formation by ectopic decorin expression. Colonies from 5 × 10⁴ freshly infected PD100 thymic lymphoma cells were grown in methyl cellulose in the presence of puromycin (2 μg/ml) and scored 12 days later. Mock, noninfected cells; LXSP, cells infected with the empty vector LXSP; LSXP-Dcn, cells infected with the retrovirus carrying the full-length mouse decorin cDNA. Error bars indicate ±SD of the mean of four independent experiments performed in duplicate. Notice the marked suppression of colony-forming ability in the decorin-expressing clones.