Table 1.
Rate constants of ‘open to closed' and ‘closed to open' conformational changes of GroEL with increasing nucleotide concentration
| Nucleotide | Concentration (μM) | kopen → closed (s−1, mean±s.d.) | kclosed → open (s−1, mean±s.d.) | Open conformation (%, mean±s.d.) |
|---|---|---|---|---|
| ADP | 10 | 1.05±0.20 | <0.1 | 1±1 |
| 30 | 1.11±0.25 | 0.10±0.03 | 7±1 | |
| 50 | 0.96±0.08 | 0.42±0.02 | 18±3 | |
| ATP | 1 | 0.71±0.21 | <0.1 | 1±1 |
| 3 | 0.68±0.06 | 0.12±0.05 | 14±1 | |
| 5 | 0.52±0.06 | 0.37±0.23 | 31±6 | |
| 7 | — | 0.63±0.16 | 40±9 | |
| ATPγS | 3 | 0.45±0.12 | <0.1 | 1±0 |
| 5 | 0.41±0.06 | <0.1 | 11±3 | |
| 7 | 0.30±0.05 | 0.26±0.08 | 30±4 | |
| ADP+AlFx | 50 | 0.52±0.02 | 0.35±0.04 | 19±4 |
| AMP-PNP | 400 | 0.34±0.02 | 0.12±0.02 | 32±4 |
| The values of the rate constants are the mean±s.d. of 3–5 independent experiments. | ||||