Figure 1.

Schematic representation of the reporter construct used in the splicing assay. Structure of the chimeric minigene used in all the transfection experiments. Patterned and white boxes indicate the pSPL3 HIV-tat exonic sequences and the human hMLH1/hMSH2 sequences. Arrows show the primers used in the RT-PCR experiments. The indicated XhoI and BamHI sites are those used to clone all the constructs. MCS = multi cloning site, Amp = ampicillin resistance gene, M = mutated construct, Wt = corresponding normal exon.