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. 2006 Aug 8;7:199. doi: 10.1186/1471-2164-7-199

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Copyright © 2006 Hass-Jacobus et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Distribution of mismatches between rice overgos and Sorghum sequence. The percentage of 36 bp overgo probes having 0 to >6 mismatches to available Sorghum sequence is shown. Sorghum sequences corresponding to regions of rice from which overgo probes were designed were identified using BLAT alignment data available from the Gramene (version 19) database [33]. Overgo sequences were aligned to Sorghum sequence by BLASTN using an open-gap cost of two and a gap-extension cost of one, with low-complexity filtration turned off.