Figure 1.
IL-10 induces CCL18 secretion by monocyte derived dendritic cells. Panel A depicts CCL18 secretion by MoDC (cultured with IL-4 and GM-CSF) upon stimulation with TNF-α, IL-1β, IL-10, IL-13, IL-15, IL-17, IL-18 (all 20 ng/ml) and IFN-γ (10 ng/ml) (n = 6). Panel B depicts CCL18 secretion by MoDC upon stimulation with LPS (2 μg/ml), CD40L or RANKL (20 ng/ml) (n = 3). Panel C depicts CCL18 secretion upon stimulation upon TLR2 (pam3cys, 10 μg/ml), TLR3 (poly (i:c), 25 μg/ml), TLR4 (LPS, 2 μg/ml) or TLR7/8 (R848, 1 μg/ml) mediated stimulation (n = 5) In all experiments, a direct comparison was made with unstimulated cells. The bars represent the mean (± SEM) CCL18 secretion in pg/ml. * represents a p-value of <0,05 (Wilcoxon Signed Rank test)