Figure 4.

Synergistic CCL18 production by monocytes upon stimulation with IL-4 and IL-10 can be induced rapidly and by low concentrations of IL-10. Panel A depicts CCL18 secretion by monocytes upon stimulation with different doses of IL-10 in the presence of IL-4. Panel B represents CCL18 secretion by monocytes that were cultured for three days in the presence or absence of IL-4 in teflon bags or rotation discs to prevent adherence. Panel C depicts CCL18 secretion by monocytes that were cultured for 24 hours with no stimulation or in the presence of IL-4, IL-13, IL-10 or a combination of IL-4/IL-13 with IL-10. The bars in panel A and B represent the mean CCL18 (pg/ml) of duplicates of 1 individual experiment and panel C shows the mean (± SEM) of 3 separate experiments. ND = not detectable