Figure 5.

The effect of antagonistic analogs of GHRH on IGF-II mRNA expression in cultured human cancer cell lines. The cells were exposed to the analogs (MZ4 = MZ-4-71, MZ5 = MZ-5-156) at 3 μM concentration for 4 hours. mRNA was extracted from the cells and amplified by using RT-PCR. After electrophoresis in 2% agarose gel, the product was stained with ethidium bromide. For internal control, GAPDH-mRNA was obtained and visualized in the same way from the RNA extracts. The PCR products were of the expected size (538 bp for IGF-II and 207 bp for GAPDH). C, control cells; M, pUC18/MspI-digested cDNA marker.