Table 2.

The effect of antagonistic analogs of GHRH on IGF-II mRNA expression of human cancer cell lines

Cell lines	Control, %	MZ-4-71, %	MZ-5-156, %
Capan-2	100	83	65
H-23	100	93	91
H-69	100	95	96
MiaPaCa-2	100	80	70
MDA-MB-468	100	59	58
OV-1063	100	98	73
PC-3	100	70	77
SW-1990	100	92	86

The cells were exposed to 3 μM concentration of analogs MZ-4-71 or MZ-5-156 for 4 hours followed by semiquantitative multiplex RT-PCR and electrophoresis on polyacrylamide gel. Optical densities of silver-stained IGF-II and hGAPDH cDNA bands were measured. Data for IGF-II bands were corrected by their hGAPDH values. Relative corrected IGF-II band densities compared to those of the control group are given in %.