Figure 4. Mating scheme to normalize Rai1 and the correlation of Rai1 copy number changes and body weight.

(A) Expected genotypes of the progeny from the mating of Dp(11)17/+ and Rai1^+/– mice. Rai1 alleles are represented by a line with circles on it. The filled circles represent normal Rai1 gene, whereas the open circles represent the null copy of Rai1. Rai1 gene copy number is further indicated in brackets for each genotype. The 4 different genotypes were obtained at expected Mendelian ratios. Mice for each of the 4 genotypes were weighed every 2 weeks until 6 months of age. (B) Weight curves for female wild-type (open diamonds), Dp(11)17/+ (filled squares), Dp(11)17/Rai1^–(open triangles), and Rai1^+/–(filled circles) mice. (C) Data for male mice are shown. Each point represents the average of weights from at least 10 mice, and error bars are indicated. *P < 0.05.