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Reactivity of mAb 56–61 with recombinant wild-type TTR and a panel of mutant TTR molecules. (A) Direct ELISA, with which the plates were coated with the TTR variants indicated. (B) Sandwich ELISA, with which the plates were coated with a rabbit polyclonal anti-TTR antibody (Dako). Binding of mAb 56–61 was demonstrated by using a horseradish peroxidase-conjugated rabbit anti-mouse antibody.
