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. 1999 Mar 16;96(6):3108–3113. doi: 10.1073/pnas.96.6.3108

Figure 2.

Figure 2

The amyloidogenic mutant TTRs53–55 but not wild-type TTR inhibits interaction between mAb 56–61 (A) or mAb 39–44 (B) and TTR coated on a plastic surface. ○, Wild-type TTR; ■, TTRs53–55. Microtiter plates were coated with 5 mg/ml wild-type TTR in PBS overnight at 4°C and then blocked with 5% skimmed milk. The mAbs and dilutions of TTR were preincubated at 37°C for 1 h in plates blocked with 5% milk, then transferred to plates coated with wild-type TTR, and incubated for 1 h. Bound mAb was visualized with horseradish peroxidase-conjugated anti-mouse IgG antibodies.