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. 1999 Mar 16;96(6):3108–3113. doi: 10.1073/pnas.96.6.3108

Figure 3.

Figure 3

Immunoreactivity of size variants of the TTRs53–55 mutant separated by size-exclusion HPLC. Size-exclusion HPLC of TTRs53–55 mutant freshly prepared (A) or aggregated in 37°C for 24 h (B). Immunoblot from native PAGE of size-exclusion HPLC fractions with polyclonal antibody (C), mAb 56–61 (D), or mAb 39–44 (E). Lanes 1–4 and 6–8 correspond to HPLC fractions as indicated; lane 5 was loaded with wild-type TTR. Lanes: 1–4, fresh material; 6–8, aggregated TTR. Arrows indicate the position of TTR tetramer; OVA (ovalbumin) and BSA indicate size markers.