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. 1999 Mar 16;96(6):3114–3119. doi: 10.1073/pnas.96.6.3114

Figure 5.

Figure 5

Androgen regulation of prostase expression. (A) Northern analysis by using a prostase probe with RNA extracted from normal prostate (NP), LNCaP at steady state (SS), LNCaP after 24 hr of androgen deprivation (0), LNCaP at specified hours after androgen exposure (1, 2, 4, 8, 24, and 48), the PC3 (PC3) and DU145 (DU145) prostate cancer cell lines, the androgen dependent (PXe-AD) and androgen independent (PXe-AI) prostate cancer xenografts, and prostate stroma (PS). A probe for β-actin is used to estimate RNA loading. (B) Relative prostase expression levels. Expression ratios are determined by normalizing all samples for β-actin intensity relative to the LNCaP steady-state (SS) sample. Prostase expression for each sample is shown in PhosphorImager intensity units relative to the SS LNCaP sample. (C) Northern analysis of prostase expression in LNCaP cells grown either with (+) or without (−) androgen (1 nM R1881) for 72 or 144 hr. A probe for β-actin is used to estimate RNA loading.