Figure 4.

Quantification of duodenal DMT1 expression in HFE^+/+ and HFE^−/− mice. RNA blot analyses were performed on poly(A)⁺ RNA from duodenum of 11 4-week-old HFE^+/+ mice and 14 HFE^−/− littermates by using the DMT1(total) probe. Signals obtained from predominant 4.4-kb transcript were quantified by phosphorimagery and normalized to that obtained by rehybridizing the blots with a probe for GAPDH. Data are presented as mean ± SEM and expressed relative to the mean value obtained from the HFE^+/+ mice. ∗, P = 0.01.