Figure 1.
Enhanced expression of MSP2 in tick salivary glands after transmission feeding. Equal numbers of A. marginale (104 per lane) from D. andersoni salivary glands obtained either before (lane 2) or 3 days after (lane 3) transmission feeding were reacted with rabbit antisera against MSP2. Salivary glands from uninfected ticks before (lane 1) or after (lane 4) 3 days of feeding were included as negative controls. Molecular size markers (kDa) are shown in the right margin. MSP2–P1 and MSP2–P2 indicate the positions of polypeptides specifically detected by the antibody to MSP2.