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. 1999 Mar 16;96(6):3194–3199. doi: 10.1073/pnas.96.6.3194

Figure 1.

Figure 1

A BL transgene is expressed in OE and VNO neurons in PompBL transgenic mice. (A) PompBL, in which the coding region of the rat OMP gene was replaced by a BL cDNA fragment (A, AatII; B, BamHI; E, EcoRI; H, HindIII; and K, KpnI). (B) Mice carrying the PompBL transgene were identified by hybridizing tail DNAs digested with BamHI to a BL cDNA probe (NT, nontransgenic; size markers in kb). BL immunoreactivity was seen in the OE (C) and VNO (F) of a neonatal L20 PompBL mouse, but not in the OE (E) or VNO (H) of a nontransgenic mouse. The BL staining patterns resembled OMP staining patterns in the OE (D) and VNO (G), except that immature neurons located in the middle of the OE were BL+, but OMP−. Radioactive BL (I) and OMP (J) cRNA probes both hybridized to OE neurons in a 3-week-old L20 mouse, but not to cells in the adjacent respiratory epithelium. No hybridization to the BL probe was seen in the OE of a nontransgenic mouse (K). [Bar = 17 μm (C–H) and 50 μm (I–K).]