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. 1999 Mar 16;96(6):3194–3199. doi: 10.1073/pnas.96.6.3194

Figure 2.

Figure 2

BL is transneuronally transferred from sensory axons to olfactory bulb neurons. In adjacent coronal olfactory bulb sections from a L20 transgenic mouse, glomeruli containing OE or VNO axons were immunoreactive for BL (A, D, and E) and OMP (B and F), as shown at low (A and B) and high (D–F) magnification. In contrast, mitral cells underlying the MOB (arrow in A and D) and AOB (arrowhead in A and E) were BL+, but OMP− (B and F). No BL labeling was seen in the olfactory bulb of a nontransgenic mouse (C). No difference was seen in olfactory bulb sections from L20 transgenic (G) versus nontransgenic (H) mice that were hybridized to a radioactive BL probe. Nasal irrigation with zinc sulfate (I) to ablate OE neurons caused a drastic reduction in BL immunoreactivity in glomeruli and mitral cells in the MOB (arrow), but not in the AOB (arrowhead), whereas nasal irrigation with saline (J) did not. [Bar = 200 μm (A–C), 25 μm (D–F), 200 μm (G and H), and 100 μm (I and J).]