Figure 3.

Quantitation of activity-dependent uptake of FM1-43 in excised living motor-nerve terminals from control and mouse LAL injected with BoTx/A. The first two bars represent measured fluorescence levels in stimulated (S) and nonstimulated (NS) terminals of control muscle. The next five bars show the progressive increase of depolarization-dependent uptake of FM1-43 into the terminals and sprouts in previously paralyzed tissue. The control level is reached only at d97. Values presented represent the mean index of FM1-43 uptake ±SEM of 100–200 measurements performed on two to six neuromuscular preparations. ∗, P < 0.01; Student’s t test.