Figure 3.
Mutation of either the dileucine- or the tyrosine-based motifs affect the TGN localization of CD25 chimeras. Forty-eight hours after transfection with the appropriate chimera cDNA, cells were treated with cycloheximide for 3 hours prior to fixation and permeabilization. Co-stainings of MPR46 and chimeras bearing either (A) the Y23S or the Y35S mutation, (B) the L3S mutation, or (C) both L3S and Y23S mutations.